Troubleshooting fine-tuning procedures for qPCR system design
نویسندگان
چکیده
منابع مشابه
Troubleshooting fine-tuning procedures for qPCR system design.
Quantitative real-time PCR (qPCR) has been improved and optimized over the past decade for a wide range of applications. Design of primers and probes is one of the crucial steps to obtain high system efficiency of qPCR since design pitfalls influence negatively amplification performances. We report the results of some experiments. First, we demonstrate the utility of optimal primer design and c...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2011
ISSN: 0887-8013
DOI: 10.1002/jcla.20489